Preliminary study on egg preservation fluid of Siberian Sturgeon, a rare fish in Yangtze River
长江珍稀鱼类西伯利亚鲟的卵子保存液的初步研究

摘要

试验对西伯利亚鲟的卵子进行保存技术研究。将参数具体设置为卵子受精率，主要研究各种类型、不同用量的保存剂、稀释液、以及根据不同的稀释程度，对实验鱼类卵子保存的实验效果。研究表明，西伯利亚鲟的卵子保存液由以下各部分组成培养液60%~70%、营养液15%~20%、鱼卵巢液9%~23%和抗菌液1%~2%。其中，甘氨酸0.5~0.6g/L、葡萄糖1.0~2.0g/L、HEPES0.8~1.2g/L、磷酸二氢钠0.2~0.4g/L、氯化钾0.17~0.38g/L、硫酸镁0.2~0.23g/L、氯化钠5.0~7.25g/L、氯化钙0.11~0.13g/L、碳酸氢钠2.0~2.5g/L，牛血清白蛋白0.5~1.0g/L，青霉素30~35万IU/L，硫酸链霉素0.2~0.4g/L。最佳卵子保存量为1毫升/200粒。最佳卵子保存液按体积分数计其包含以下各组分：培养液65%、营养液17.5%、鱼卵巢液16%和抗菌液1.5%。此次研究结果可以为日后保存长江珍稀鲟属鱼类的种质资源奠定了坚实的研究基础。

Abstract

In this study, the egg preservation techniques of Siberian sturgeon were studied. The parameters were specifically set as egg fertilization rate, and the experimental effects of various types, different amounts of preservatives, diluents, and different dilution degrees on egg preservation of experimental fish were mainly studied. Studies have shown that the egg preservation solution of Siberian sturgeon is composed of the following parts: culture solution 60%~70%, nutrient solution 15%~20%, fish ovarian fluid 9%~23% and antibacterial solution 1%~2%. Among them, Glycine 0.5~0.6g/L, glucose 1.0~2.0g/L, HEPES0.8~1.2g/L, sodium dihydrogen phosphate 0.2~0.4g/L, potassium chloride 0.17~0.38g/L, magnesium sulfate 0.2~0.23g/L, sodium chloride 5.0~7.25g/L, calcium chloride 0.11~0.13g/ L L, sodium bicarbonate 2.0~2.5g/L, bovine serum albumin 0.5~1.0g/L, penicillin 300 ~ 350,000 IU/L, streptomycin sulfate 0.2~0.4g/L. The optimal egg storage is 1 ml /200 eggs. The optimal egg preservation solution by volume consists of the following components: culture solution 65%, nutrient solution 17.5%, fish ovarian solution 16%, and antibacterial solution 1.5%. The results of this study can lay a solid foundation for the future conservation of the germplasm resources of rare acipenser in the Yangtze River.