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当前位置：现代生命科学研究 > 2025年 6卷 (1)期

Open Access Article

Journal of Modern Life Sciences Research. 2025; 6: (1) ; 32-41 ; DOI: 10.12208/j.jlsr.20250005.

Identification and expression analysis of the abscisic Acid 8'-Hydroxylase (ABAH) gene family in Citrus grandis ‘Tomentosa’
化橘红脱落酸8'-羟化酶基因家族的鉴定及其表达分析

作者： 吴佳欣^1,2,3, 张旭敏¹, 曹征⁴, 冯伟彬⁴, 沈超¹ *

1 广东石油化工学院生物与食品工程学院广东茂名

2 喀什大学生命与地理科学学院新疆喀什

3 新疆帕米尔高原生物资源与生态重点实验室新疆喀什

4 茂名市农业科技推广中心广东茂名

*通讯作者：沈超,单位：广东石油化工学院生物与食品工程学院广东茂名；

茂名市科技计划项目（2021KJZXZJGSPDX006）广东石油化工学院人才引进项目（2019rc112）广东石油化工学院教育教学改革项目（710136090405）

发布时间: 2025-06-30 总浏览量: 16

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摘要

8'-羟化酶（ABAH）是植物脱落酸（ABA）失活代谢的关键酶，参与调控植物生长发育及逆境胁迫响应。为解析化橘红（Citrus grandis ‘Tomentosa’）中ABAH基因家族的组成与功能，本研究在全基因组水平系统鉴定出6个ABAH家族成员。结构预测结果显示，该家族成员具有高度保守的二级与三级结构，暗示其功能在进化过程中相对稳定。系统进化分析发现，其中Cmt252050基因单独成簇，与拟南芥ABAH成员亲缘关系较远，可能具有独特的生物学功能。启动子顺式元件分析表明，大多数成员富含ABA响应元件，提示其在ABA信号调控网络中可能发挥作用。结合化橘红果实发育阶段的转录组数据，发现Cmt252050在内果皮发育后期显著高表达，推测其可能参与调控果皮ABA积累及发育过程。本研究为深入揭示ABAH基因在化橘红果皮发育中的作用及ABA代谢调控机制提供了理论依据与候选基因资源。

关键词： 化橘红；8'-羟化酶；ABA代谢；果皮发育；基因表达分析

Abstract

8'-Hydroxylase (ABAH) is a key enzyme in the oxidative catabolism of abscisic acid (ABA), playing essential roles in plant development and stress responses. In this study, six ABAH gene family members were systematically identified in Citrus grandis ‘Tomentosa’ (Huajuhong). Structural predictions revealed that these proteins possess highly conserved secondary and tertiary structures, suggesting functional conservation during evolution. Phylogenetic analysis indicated that one member, Cmt252050, forms an independent clade and is distantly related to ABAH genes in Arabidopsis thaliana, implying possible species-specific functions. Promoter analysis showed that ABA-responsive cis-elements are widely distributed in this gene family, highlighting their potential involvement in ABA signaling pathways. Transcriptome profiling during fruit development revealed that Cmt252050 is highly expressed in the later stages of inner pericarp development, suggesting a role in ABA accumulation and pericarp regulation. This study provides foundational insights into the ABAH gene family in C. grandis and offers valuable candidate genes for further investigation of ABA metabolism and fruit development mechanisms in Huajuhong.

Key words： Citrus grandis ‘Tomentosa’; 8'-hydroxylase; ABA metabolism; Pericarp development; Gene expression analysis

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引用本文

吴佳欣, 张旭敏, 曹征, 冯伟彬, 沈超, 化橘红脱落酸8'-羟化酶基因家族的鉴定及其表达分析[J]. 现代生命科学研究, 2025; 6: (1) : 32-41.

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